229e n his (Sino Biological)

Sino Biological is a verified supplier

Sino Biological manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Sino Biological 229e n his
229e N His, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/229e+n+his/Human+coronavirus+(HCoV-229E)+Nucleocapsid+Protein/pm37399385-346-39-42
Average 93 stars, based on 29 article reviews

229e n his - by Bioz Stars, 2026-09

93/100 stars

Images

Recombinant:

Article Title: Cell Surface Nucleocapsid Protein Expression: A Betacoronavirus Immunomodulatory Strategy
Article Snippet: The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2.5 h at 37° C with 5% CO 2 , measuring absorbance at 490 nm using a Synergy H1 plate reader (Bio-Tek). .. The following recombinant proteins were used: SARS-CoV-2 N-His (Sino Biological # 40588-V07E), HCoV-OC43 N-His (Sino Biological # 40643-V07E), GFP-His (Thermo Fisher # A42613), NL63 N-His (Sino Biological # 40641-V07E), 229E N-His (Sino Biological # 40640-V07E), and HKU1 N-His (Sino Biological # 40642-V07E). .. For each indicated cell type, 2.5 x 10 4 cells were seeded on 96-well flat white tissue culture-treated plates (Thermo Fisher # 136101), cultured overnight, and infected with HCoV-OC43 at an MOI of 1 PFU/cell (target cells) at 35° C. At 24 hpi, infected target cells were washed with DPBS and the medium was replaced with 40 μl of RPMI 1640 with 4% low IgG serum (Promega # G711A) containing 5 x 10 4 Jurkat effector cells (Promega # G701A) and 10 μl of pooled mouse sera from naïve or immunized mice with HCoV-OC43 N-His.

Article Title: Cell Surface Nucleocapsid Protein Expression: A Betacoronavirus Immunomodulatory Strategy
Article Snippet: The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2.5 h at 37° C with 5% CO 2 , measuring absorbance at 490 nm using a Synergy H1 plate reader (Bio-Tek). .. The following recombinant proteins were used: SARS-CoV-2 N-His (Sino Biological # 40588-V07E), HCoV-OC43 N-His (Sino Biological # 40643-V07E), GFP-His (Thermo Fisher # A42613), NL63 N-His (Sino Biological # 40641-V07E), 229E N-His (Sino Biological # 40640-V07E), and HKU1 N-His (Sino Biological # 40642-V07E). .. For each indicated cell type, 2.5 × 10 4 cells were seeded on 96-well flat white tissue culture-treated plates (Thermo Fisher # 136101), cultured overnight, and infected with HCoV-OC43 at an MOI of 1 PFU/cell (target cells) at 35° C. At 24 hpi, infected target cells were washed with DPBS and the medium was replaced with 40 μl of RPMI 1640 with 4% low IgG serum (Promega # G711A) containing 5 × 10 4 Jurkat effector cells (Promega # G701A) and 10 μl of pooled mouse sera from naïve or immunized mice with HCoV-OC43 N-His.

Article Title: Cell surface nucleocapsid protein expression: A betacoronavirus immunomodulatory strategy.
Article Snippet: The migrated cells in the lower chamber were stained with 5 μL CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2.5 h at 37 °C with 5% CO2, measuring absorbance at 490 nm using a Synergy H1 plate reader (BioTek). .. The following recombinant proteins were used: SARS- CoV- 2 N- His (Sino Biological # 40588- V07E), HCoV- OC43 N- His (Sino Biological # 40643- V07E), GFP- His (Thermo Fisher # A42613), NL63 N- His (Sino Biological # 40641- V07E), 229E N- His (Sino Biological # 40640- V07E), and HKU1 N- His (Sino Biological # 40642- V07E). ..

Review

229e n his (Sino Biological)

Structured Review

Images

Related Articles

Recombinant:

Similar Products

Image Search Results